《植物生理学报》 2012, 48(4): 325-332

桉树胚状体再生与遗传转化的研究进展

沙月娥^1,2, 欧阳乐军^1,2, 彭舒^1,2, 黄真池¹, 陈信波², 曾富华^1,*

湛江师范学院生命科学与技术学院, 广东湛江524048; 2湖南农业大学生物科学技术学院, 长沙410128

通信作者：曾富华；E-mail: zengfuhua@gmail.com；Tel: 0759-3183622

摘　要：

文章综述了桉树胚状体发生过程中基因型、外植体材料、培养基、光照、植物生长调节剂种类与配比以及其他添加物对外植体胚性愈伤组织诱导以及胚状体发生的影响, 探讨了影响桉树遗传转化体系建立的因素, 并对近年来桉树胚状体再生和转基因研究进展进行了介绍

关键词：桉树; 胚状体; 遗传转化; 植株再生

收稿：2011-12-16   修定：2012-02-29

资助：国家星火计划项目(2011GA780067)、广东省自然科学基金(10152404801000005和10452404801004328)、广东省科技攻关项目(2010B020301011)和湛江市科技攻关项目(2011C3104010)。

Research Progress in Plantlet Regeneration of Somatic Embryos and Genetic Transformation in Eucalyptus

SHA Yue-E^1,2, OUYANG Le-Jun^1,2, PENG Shu^1,2, HUANG Zhen-Chi¹, CHEN Xin-Bo², ZENG Fu-Hua^1,*

¹School of Life Science and Technology, Zhanjiang Normal University, Zhanjiang, Guangdong 524048, China;²College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

Corresponding author: ZENG Fu-Hua; E-mail: zengfuhua@gmail.com; Tel: 0759-3183622

Abstract:

An overview is made on somatic embryogenesis and genetic transformation of Eucalyptus in the paper. The impact of the genotype, explant material, media, light, plant growth regulators and other additives on embryogenic callus inducing and somatic embryogenesis of Eucalyptus is discussed. A few of the factors which affect the establishment of genetic transformation system of Eucalyptus are studied. Researches of somatic embryogenesis and genetic transformation of Eucalyptus in recent years are also introduced.

Key words: Eucalyptus; somatic embryos; genetic transformation; plantlet regeneration